The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 05, 2023
Filed:
Oct. 16, 2020
Applicants:
Riken, Wako, JP;
Sumitomo Chemical Company, Limited, Tokyo, JP;
Inventors:
Yoshiki Sasai, Wako, JP;
Hidetaka Suga, Wako, JP;
Assignees:
RIKEN, Wako, JP;
Sumitomo Chemical Company, Limited, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01); C12N 5/071 (2010.01); A61K 35/30 (2015.01); A61K 35/55 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0618 (2013.01); A61K 35/30 (2013.01); A61K 35/55 (2013.01); C12N 5/0613 (2013.01); C12N 5/0616 (2013.01); C12N 2501/33 (2013.01); C12N 2501/39 (2013.01); C12N 2501/392 (2013.01); C12N 2501/41 (2013.01); C12N 2501/42 (2013.01); C12N 2506/02 (2013.01);
Abstract
The present invention provides a method of obtaining aggregates containing a rostral hypothalamus tissue and a rostral head ectodermal tissue, a hypophysis precursor tissue and a hypophysis hormone producing cell, by using a serum-free medium (preferably substantially free of growth factor and insulins), forming homogeneous aggregates of stem cells from pluripotent stem cells such as ES cell and the like, which are plated at a high cell concentration, and subjecting the formed aggregates to floating-culture.