The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 05, 2023

Filed:

Apr. 13, 2020
Applicants:

The Regents of the University of California, Oakland, CA (US);

Pioneer Hi-bred International, Inc., Johnston, IA (US);

Inventors:

Abraham P. Lee, Irvine, CA (US);

Michelle A. Digman, Irvine, CA (US);

Dohyun Lee, Irvine, CA (US);

Xuan Li, Irvine, CA (US);

Ning Ma, Irvine, CA (US);

Yue Yun, Johnston, IA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/02 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); B01L 3/502715 (2013.01); C12N 15/1003 (2013.01); C12Q 1/02 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0864 (2013.01);
Abstract

Methods and devices for single cell analysis using fluorescence lifetime imaging microscopy (FLIM) are disclosed. The methods utilize microfluidic devices which use traps to immobilize cells for FLIM analysis. The analysed cells may be sorted before or after imaging and may be plant, animal, or bacterial cells. Analysis of the FLIM data may use a phasor plot and may be used to identify a metabolic pattern of the single cells.


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