The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2023
Filed:
Jun. 21, 2021
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Kevin Aliado, Sunnyvale, CA (US);
Roger J. A. Chen, Saratoga, CA (US);
Jing Luo, Daly City, CA (US);
J. William Maney, Jr., Emerald Hills, CA (US);
William Nielsen, San Jose, CA (US);
Kyle Umeda, Saratoga, CA (US);
Ashraf Wahba, Hayward, CA (US);
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Abstract
A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.