The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2023

Filed:

Apr. 02, 2019
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Richard Novak, Boston, MA (US);

Sasan Jalili-Firoozinezhad, Cambridge, MA (US);

Francesca S. Gazzaniga, Jamaica Plain, MA (US);

Elizabeth L. Calamari, Ashland, MA (US);

Diogo M. Camacho, Framingham, MA (US);

Bret A. Nestor, Regina, CA;

Cicely Fadel, Brookline, MA (US);

Michael L. Cronce, Roxbury, MA (US);

Dennis L. Kasper, Boston, MA (US);

Donald E. Ingber, Boston, MA (US);

Amir Bein, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12M 3/06 (2006.01); C12N 1/20 (2006.01); C12M 3/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0679 (2013.01); C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12N 1/20 (2013.01);
Abstract

A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.


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