The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2023
Filed:
Mar. 02, 2020
Cansino Biologics Inc., Tianjin, CN;
Tao Zhu, Tianjin, CN;
Haiyan Cui, Tianjin, CN;
Weiwei Chen, Tianjin, CN;
Lei Duan, Tianjin, CN;
Junqiang Li, Tianjin, CN;
Jin Ma, Tianjin, CN;
Chunlin Xin, Tianjin, CN;
Zhongqi Shao, Tianjin, CN;
Xuefeng Yu, Tianjin, CN;
Huihua Mao, Tianjin, CN;
CANSINO BIOLOGICS INC., Tianjin, CN;
Abstract
Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace said gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.