The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 2023

Filed:

Oct. 09, 2018
Applicants:

Hiroshima University, Higashi-Hiroshima, JP;

Repertoire Genesis Incorporation, Ibaraki, JP;

Inventors:

Tatsuo Ichinohe, Hiroshima, JP;

Takashi Yamamoto, Higashi-Hiroshima, JP;

Tetsushi Sakuma, Higashi-Hiroshima, JP;

Yasuko Honjo, Hiroshima, JP;

Takakazu Kawase, Hiroshima, JP;

Takahiko Miyama, Hiroshima, JP;

Ryuji Suzuki, Ibaraki, JP;

Assignees:

Hiroshima University, Higashi-Hiroshima, JP;

Repertoire Genesis Incorporation, Ibaraki, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 15/64 (2006.01); C07K 19/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 15/64 (2013.01); C07K 19/00 (2013.01); C07K 2319/81 (2013.01);
Abstract

The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.


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