The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 2023

Filed:

Mar. 14, 2018
Applicant:

Cedars-sinai Medical Center, Los Angeles, CA (US);

Inventors:

Dhruv Sareen, Porter Ranch, CA (US);

Berhan Mandefro, Sherman Oaks, CA (US);

Anjoscha Kaus, Los Angeles, CA (US);

Assignee:

Cedars-Sinai Medical Center, Los Angeles, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C12N 5/074 (2010.01); C12N 5/073 (2010.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C12N 5/0793 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0658 (2013.01); C12N 5/0068 (2013.01); C12N 5/0603 (2013.01); C12N 5/0619 (2013.01); C12N 5/0696 (2013.01); C12N 5/0697 (2013.01); C12N 2501/105 (2013.01); C12N 2501/115 (2013.01); C12N 2501/12 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/50 (2013.01);
Abstract

The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).


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