The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 29, 2023
Filed:
Mar. 18, 2020
Arcturus Therapeutics, Inc., San Diego, CA (US);
Priya Karmali, San Diego, CA (US);
Padmanabh Chivukula, San Diego, CA (US);
Joseph E. Payne, San Diego, CA (US);
Yanjie Bao, San Diego, CA (US);
Arcturus Therapeutics, Inc., San Diego, CA (US);
Abstract
A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1tube having an inner diameter (ID) of between about 0.1″ and 0.132″; b) flowing an ethanol solution comprising lipids through a 2tube having an ID of between about 0.005″ and 0.02″ at one third the flow rate of the aqueous solution through the 1tube, wherein the lipids comprise a cationic lipid; and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2tube perpendicularly joined to the 1tube; wherein the mixing produces an output solution flowing in the 1tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.