The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 22, 2023
Filed:
Sep. 28, 2022
New England Biolabs, Inc., Ipswich, MA (US);
Nathan Tanner, West Newbury, MA (US);
Yinhua Zhang, North Reading, MA (US);
Eric Hunt, Danvers, MA (US);
Gregory Patton, Peabody, MA (US);
Guoping Ren, Danvers, MA (US);
Zhiru Li, Lexington, MA (US);
Andrew Barry, Ipswich, MA (US);
Nicole Nichols, Reading, MA (US);
Catherine B. Poole, Medford, MA (US);
Harriet M. Strimpel, Manchester, MA (US);
Ivan R. Correa, Jr., Hamilton, MA (US);
Clotilde Carlow, Ipswich, MA (US);
Esta Slayton, Epping, NH (US);
Thomas C. Evans, Jr., Topsfield, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.