The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 15, 2023
Filed:
Jun. 24, 2016
Applicant:
Dana-farber Cancer Institute, Inc., Boston, MA (US);
Inventors:
Gerassimos Makrigiorgos, Chestnut Hill, MA (US);
Chen Song, Boston, MA (US);
Assignee:
Dana-Farber Cancer Institute, Inc., Boston, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12N 9/22 (2006.01); C12Q 1/6883 (2018.01); C12N 9/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 9/22 (2013.01); C12N 15/10 (2013.01); C12N 15/1093 (2013.01); C12P 19/34 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6883 (2013.01);
Abstract
The present disclosure provides methods for preparing a target mutant nucleic acid for subsequent enrichment relative to a wild type nucleic acid using nucleases that have a substantially higher activity on double stranded DNA versus single stranded DNA or RNA. The present disclosure also includes methods for enriching a target mutant nucleic acid and for preparing unmethylated/methylated nucleic acids of interest for subsequent enrichment.