The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 07, 2023

Filed:

Mar. 08, 2017
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Aviv Regev, Cambridge, MA (US);

Evan Zane Macosko, Cambridge, MA (US);

Steven Andrew McCarroll, Cambridge, MA (US);

Alexander K. Shalek, Cambridge, MA (US);

Anindita Basu, Cambridge, MA (US);

Christopher B. Ford, Cambridge, MA (US);

Hongkun Park, Lexington, MA (US);

David A. Weitz, Bolton, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6809 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6869 (2018.01); C12N 15/10 (2006.01); G06K 19/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6809 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6869 (2013.01); G06K 19/06 (2013.01);
Abstract

The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.


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