The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 07, 2023

Filed:

Oct. 03, 2019
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

University of Tokyo, Tokyo, JP;

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Silvana Konermann, Zurich, CH;

Alexandro Trevino, Stanford, CA (US);

Mark Brigham, Somerville, MA (US);

Fei Ran, Boston, MA (US);

Patrick Hsu, San Diego, CA (US);

Chie-yu Lin, Boston, MA (US);

Osamu Nureki, Kanagawa, JP;

Hiroshi Nishimasu, Tokyo, JP;

Ryuichiro Ishitani, Tokyo, JP;

Feng Zhang, Cambridge, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); A61K 38/46 (2006.01); A61K 48/00 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01); C12Q 1/6876 (2018.01); A61P 43/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); A61K 38/465 (2013.01); A61K 48/005 (2013.01); C12N 15/113 (2013.01); C12N 15/902 (2013.01); C12N 15/907 (2013.01); C12Q 1/6876 (2013.01); C12Y 301/00 (2013.01); C12N 2310/20 (2017.05);
Abstract

The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.


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