The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 2023
Filed:
May. 27, 2016
Applicant:
Boehringer Ingelheim International Gmbh, Ingelheim am Rhein, DE;
Inventors:
Gregory Walter Hiller, Wakefield, MA (US);
Matthew Paul Gagnon, Medford, MA (US);
Ana Maria Ovalle, Medford, MA (US);
Assignee:
Boehringer Ingelheim International GmbH, Ingelheim am Rhein, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01);
U.S. Cl.
CPC ...
C12M 29/10 (2013.01); C12M 41/26 (2013.01); C12N 5/0018 (2013.01); C12N 5/0062 (2013.01); C12N 2500/60 (2013.01); C12N 2510/02 (2013.01); C12N 2511/00 (2013.01);
Abstract
Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.