The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2023
Filed:
Feb. 26, 2020
Applicant:
National University Corporation Gunma University, Gunma, JP;
Inventors:
Assignee:
NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY, Gunma, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 15/90 (2006.01); C07K 16/18 (2006.01); C12N 5/10 (2006.01); C12N 9/10 (2006.01); C12N 15/09 (2006.01); C07K 16/44 (2006.01); C12N 1/00 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); C07K 16/18 (2013.01); C07K 16/44 (2013.01); C12N 1/00 (2013.01); C12N 5/10 (2013.01); C12N 9/10 (2013.01); C12N 15/09 (2013.01); C12N 15/111 (2013.01); C07K 2317/622 (2013.01); C07K 2319/01 (2013.01); C07K 2319/21 (2013.01); C07K 2319/60 (2013.01); C07K 2319/80 (2013.01); C12N 2310/20 (2017.05); C12Y 201/01037 (2013.01);
Abstract
A DNA methylation editing kit comprises: (1) a fusion protein of inactivated CRISPR-associated endonuclease Cas9 (dCas9) having no nuclease activity and a tag peptide array in which plural tag peptides are linked by linkers, or an RNA or DNA coding therefor; (2) a fusion protein(s) of a tag peptide-binding portion and a methylase or demethylase, or an RNA(s) or DNA(s) coding therefor; and (3) a guide RNA(s) (gRNA(s)) comprising a sequence complementary to a DNA sequence within 1 kb of a desired site of methylation or demethylation, or a DNA(s) expressing the gRNA(s).