The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 24, 2023

Filed:

Apr. 08, 2020
Applicant:

Thermo Fisher Scientific Baltics Uab, Carlsbad, CA (US);

Inventors:

Remigijus Skirgaila, Vilnius, LT;

Renata Rimseliene, Vilnius, LT;

Rasa Sukackaite, Vilnius, LT;

Martyna Simutyte, Vilnius, LT;

Skaiste Valinskyte, Kazlu̧ Rūda, LT;

Karolis Matjosaitis, Vilnius, LT;

Mindaugas Ukanis, Vilnius, LT;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12N 9/1252 (2013.01); C12P 19/34 (2013.01); C12Q 1/686 (2013.01); C12Y 207/07007 (2013.01); C07K 2319/80 (2013.01);
Abstract

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3'-5′ exonuclease domain and/or a sequence non-specific dsDNA binding domain.


Find Patent Forward Citations

Loading…