The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2023
Filed:
Oct. 31, 2018
The Broad Institute, Inc., Cambridge, MA (US);
Dana-farber Cancer Institute, Inc., Boston, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Pratiti Bandopadhayay, Boston, MA (US);
Rameen Beroukhim, Boston, MA (US);
Paul Blainey, Cambridge, MA (US);
David Feldman, Cambridge, MA (US);
Cory Johannessen, Cambridge, MA (US);
Funien Tsai, Cambridge, MA (US);
THE BROAD INSTITUTE, INC., Cambridge, MA (US);
DANA-FARBER CANCER INSTITUTE, INC., Boston, MA (US);
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);
Abstract
The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence comprising a guide sequence. Barcoded cells can then be recovered using a reporter construct having CRISPR target sequences specific for the cell having a barcode of interest.