The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 13, 2022
Filed:
Mar. 21, 2022
New England Biolabs, Inc., Ipswich, MA (US);
Nathan Tanner, West Newbury, MA (US);
Yinhua Zhang, North Reading, MA (US);
Eric Hunt, Danvers, MA (US);
Gregory Patton, Peabody, MA (US);
Guoping Ren, Danvers, MA (US);
Zhiru Li, Lexington, MA (US);
Andrew Barry, Ipswich, MA (US);
Nicole Nichols, Reading, MA (US);
Catherine B. Poole, Medford, MA (US);
Harriet M. Strimpel, Manchester, MA (US);
Ivan R. Correa, Jr., Hamilton, MA (US);
Clotilde Carlow, Ipswich, MA (US);
Esta Slayton, Epping, NH (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.