The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 01, 2022

Filed:

Feb. 07, 2020
Applicant:

Nch Corporation, Irving, TX (US);

Inventors:

Gabriel F. K. Everett, Coppell, TX (US);

Charles Greenwald, Dallas, TX (US);

Judy Pruitt, Mesquite, TX (US);

Amanda Rosmarin, Lantana, TX (US);

Jordan Church, Dallas, TX (US);

Daniel Aberle, Irving, TX (US);

George Aboagye, Derby, GB;

Assignee:

NCH Corporation, Irving, TX (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/742 (2015.01); A61K 47/02 (2006.01); C12N 3/00 (2006.01); C02F 3/34 (2006.01); C12N 1/04 (2006.01); C12N 1/20 (2006.01); A23L 29/00 (2016.01); C12R 1/01 (2006.01); C12R 1/07 (2006.01); C12R 1/125 (2006.01);
U.S. Cl.
CPC ...
A61K 35/742 (2013.01); A23L 29/065 (2016.08); A61K 47/02 (2013.01); C02F 3/34 (2013.01); C02F 3/348 (2013.01); C12N 1/04 (2013.01); C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12N 3/00 (2013.01); C12R 2001/01 (2021.05); C12R 2001/07 (2021.05); C12R 2001/125 (2021.05);
Abstract

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or morespecies, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.


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