The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2022

Filed:

Jul. 10, 2020
Applicants:

Pioneer Hi-bred International, Inc., Johnston, IA (US);

E. I. Du Pont DE Nemours and Company, Wilmington, DE (US);

Inventors:

Andrew Mark Cigan, Madison, WI (US);

Saverio Carl Falco, Wilmington, DE (US);

Huirong Gao, Johnston, IA (US);

Michael Lassner, Portland, OR (US);

Derek Jantz, Durham, NC (US);

Zhongsen Li, Hockessin, DE (US);

Zhan-Bin Liu, Clive, IA (US);

Sergei Svitashev, Johnston, IA (US);

James Jefferson Smith, Durham, NC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); A01H 6/46 (2018.01); A01H 1/06 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8202 (2013.01); A01H 1/06 (2013.01); C12N 9/22 (2013.01); C12N 15/8213 (2013.01);
Abstract

Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.


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