The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 20, 2022

Filed:

Aug. 13, 2019
Applicant:

The Rockefeller University, New York, NY (US);

Inventors:

Michael P. Rout, New York, NY (US);

Brian T. Chait, New York, NY (US);

Assignee:

The Rockefeller University, New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); G16B 30/00 (2019.01); C07K 16/44 (2006.01); G01N 33/68 (2006.01); C07K 16/00 (2006.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C40B 30/04 (2013.01); C07K 16/00 (2013.01); C07K 16/44 (2013.01); C12Q 1/6876 (2013.01); G01N 33/6857 (2013.01); G16B 30/00 (2019.02); C07K 2317/22 (2013.01); C07K 2317/569 (2013.01); C07K 2317/92 (2013.01);
Abstract

Provided are compositions and methods for producing large repertoires of recombinant nanobodies with high affinities and specificities against any antigen. Included are methods for making and identifying nanobodies produced by camelids, the nanobodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the nanobodies recombinantly. Antigen-specific nanobodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided.


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