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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11447818 B1

PDFFull Text
Date of Patent:
Sep. 20, 2022

Filed:

Sep. 12, 2018

Universal short adapters with variable length non-random unique molecular identifiers

Applicant:

Illumina, Inc., San Diego, CA (US);

Inventors:

Chen Zhao, San Diego, CA (US);

Kevin Wu, San Diego, CA (US);

Han-Yu Chuang, San Diego, CA (US);

Jennifer Lococo, San Diego, CA (US);

Alex So, San Diego, CA (US);

Dwight Baker, San Diego, CA (US);

Tatjana Singer, San Diego, CA (US);

Assignee:

Illumina, Inc., San Diego, CA (US);

Attorney:

Weaver Austin Villeneuve & Sampson LLP

Primary Examiner:

Kenneth R Horlick

Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6855 (2018.01); G16B 20/20 (2019.01); G16B 35/10 (2019.01); G16B 30/10 (2019.01); G16B 25/20 (2019.01); G16B 40/00 (2019.01); G16B 20/00 (2019.01); G16B 30/00 (2019.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6855 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01); G16B 20/00 (2019.02); G16B 20/20 (2019.02); G16B 25/20 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02); G16B 35/10 (2019.02); G16B 40/00 (2019.02); C12Q 2525/191 (2013.01); C12Q 2525/197 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01);
Abstract

The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

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