The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 20, 2022

Filed:

Mar. 11, 2019
Applicants:

Keck Graduate Institute, Claremont, CA (US);

H. Lundbeck A/s, Valby, DK;

Inventors:

James M. Cregg, Claremont, CA (US);

John A. Latham, Seattle, WA (US);

Mark Litton, Seattle, WA (US);

Randall Schatzman, Redmond, WA (US);

Ilya I. Tolstrorukov, Claremont, CA (US);

Assignees:

KECK GRADUATE INSTITUTE, Claremont, CA (US);

H. LUNDBECK A/S, Valby, DK;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/28 (2006.01); C07K 16/00 (2006.01); C07K 16/18 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2896 (2013.01); C07K 16/00 (2013.01); C07K 16/18 (2013.01); C07K 16/2809 (2013.01); C12N 15/81 (2013.01); C12N 15/815 (2013.01); C07K 2317/14 (2013.01); C07K 2317/24 (2013.01);
Abstract

Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.


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