The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2022

Filed:

Mar. 15, 2019
Applicant:

John Innes Centre, Norwich, GB;

Inventors:

Sarah O'Connor, Norwich, GB;

Lorenzo Caputi, Norwich, GB;

Richard Payne, Broadstairs, GB;

Jakob Franke, Hannover, DE;

Scott Farrow, Norfolk, GB;

Assignee:

John Innes Centre, Norwich, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/18 (2006.01); C12N 15/82 (2006.01); C12R 1/19 (2006.01); C12R 1/865 (2006.01);
U.S. Cl.
CPC ...
C12P 17/182 (2013.01); C12N 15/8243 (2013.01); C12R 2001/19 (2021.05); C12R 2001/865 (2021.05); C12Y 103/01 (2013.01);
Abstract

The complex chemistry underlying the extensive transformations involved in terpene indole alkaloid synthesis makes identification of the biosynthetic genes challenging. The present invention relates to methods for producing a terpene indole alkaloid derivative, comprising the steps of: (1) providing a terpene indole alkaloid; and (2) providing a first enzyme termed 'Precondylocarpine Acetate Synthase' (PAS) or a functional variant or homologue thereof; and/or a second enzyme termed 'Dehydroprecondylocarpine Acetate Synthase' (DPAS) or a functional variant or homologue thereof, and optionally providing further identified enzymes involved in this pathway. The invention also encompasses related kits, enzymes, expression vectors, host cells and plants.


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