The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 26, 2022

Filed:

Oct. 23, 2017
Applicant:

Exosome Diagnostics, Inc., Waltham, MA (US);

Inventors:

Johan Skog, Lincoln, MA (US);

Sudipto Chakrabortty, Waltham, MA (US);

Dalin Chan, Brighton, MA (US);

Michael Valentino, Waltham, MA (US);

Vasisht Tadigotla, Newton, MA (US);

Robert Kitchen, Somerville, MA (US);

Dominik Grimm, Schondorf am Ammersee, DE;

Wei Yu, Belmont, MA (US);

Assignee:

Exosome Diagnostics, Inc., Waltham, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6874 (2018.01); C12N 15/10 (2006.01); G01N 1/28 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12N 15/1096 (2013.01); G01N 1/28 (2013.01); G01N 33/54326 (2013.01);
Abstract

The invention provides a series of steps that prepare nucleic acids (RNA and/or DNA) isolated from extracellular vesicles for sequencing. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected. These can then be used to identify various attributes such as gene expression, alternative splicing, and the detection of both somatic and germline mutations including single nucleotide variants (SNV) and structural variations (insertions/deletions, fusions, inversions).


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