The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 05, 2022

Filed:

Feb. 17, 2017
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Dong Sung An, Los Angeles, CA (US);

Saki Shimizu, Berkeley, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 5/00 (2006.01); A61K 35/28 (2015.01); A61K 45/06 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1137 (2013.01); A61K 35/28 (2013.01); A61K 45/06 (2013.01); C12N 5/0081 (2013.01); C12N 15/86 (2013.01); C12N 2310/531 (2013.01); C12N 2740/15043 (2013.01); C12Y 204/02008 (2013.01);
Abstract

A potent short hairpin RNA (shRNA734) directed to human Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) improves the rate of gene-modified stem cell engraftment by a conditioning and in vivo selection strategy to confer resistance to a clinically available guanine analog antimetabolite, 6TG, for efficient positive selection of gene-modified stem cells. Uses for polynucleotides comprising the shRNA734 include methods for knocking down HPRT in a cell, for conferring resistance to a guanine analog antimetabolite in a cell, for producing selectable genetically modified cells, for selecting cells genetically modified with a gene of interest from a plurality of cells, for removing cells genetically modified with a gene of interest from a plurality of cells, and for treating a subject infected with HIV.


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