The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2022
Filed:
Aug. 03, 2017
Applicant:
Meiragtx Uk Ii Limited, London, GB;
Inventors:
Xuecui Guo, Cold Spring Harbor, NY (US);
Alexandria Forbes, New York, NY (US);
Lei Feng, Bronx, NY (US);
Assignee:
MEIRAGTX UK II LIMITED, London, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6811 (2018.01); C12N 15/115 (2010.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6811 (2013.01); C12N 15/1048 (2013.01); C12N 15/115 (2013.01); C12N 2310/16 (2013.01); C12N 2320/11 (2013.01); C12N 2330/31 (2013.01);
Abstract
The invention provides eukaryotic cell-based screening methods to identify an aptamer that specifically binds a ligand, or a ligand that specifically binds an aptamer, using a polynucleotide cassette for the regulation of the expression of a reporter gene where the polynucleotide cassette contains a riboswitch in the context of a 5' intron-alternative exon-3′ intron. The riboswitch comprises an effector region and an aptamer such that when the aptamer binds a ligand, reporter gene expression occurs.