The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2022

Filed:

Sep. 14, 2020
Applicant:

Lonza Ltd, Visp, CH;

Inventors:

Colin Mark Jaques, Slough, GB;

Mohsan Waseem Khan, Slough, GB;

Rita D'Ornelas P. De Barros Costa, Slough, GB;

Anthony Beaney, Slough, GB;

David Valentine, Slough, GB;

Assignee:

Lonza Ltd, Visp, CH;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/06 (2006.01); B01F 23/231 (2022.01); B01F 27/053 (2022.01); B01F 27/86 (2022.01); B01F 27/191 (2022.01); B01F 27/113 (2022.01); B01F 35/513 (2022.01);
U.S. Cl.
CPC ...
C12M 23/28 (2013.01); B01F 23/231 (2022.01); B01F 27/053 (2022.01); B01F 27/1134 (2022.01); B01F 27/191 (2022.01); B01F 27/86 (2022.01); B01F 35/513 (2022.01); C12M 23/14 (2013.01); C12M 23/26 (2013.01); C12M 27/02 (2013.01); C12M 27/20 (2013.01); C12M 29/00 (2013.01); C12M 29/06 (2013.01); B01F 2215/0431 (2013.01); B01F 2215/0459 (2013.01);
Abstract

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.


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