The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 31, 2022

Filed:

Mar. 07, 2019
Applicants:

Hai Xing Chen, Toronto, CA;

Hong Yang Wang, Shanghai, CN;

Zhongtian Qi, Shanghai, CN;

Inventors:

Hai Xing Chen, Toronto, CA;

Hong Yang Wang, Shanghai, CN;

Zhongtian Qi, Shanghai, CN;

Assignee:

Other;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12N 9/22 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); C12N 9/22 (2013.01); A01K 2217/052 (2013.01); A01K 2227/101 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C12N 2015/8518 (2013.01);
Abstract

A method of producing a non-erythroid protein in erythrocytes of a transgenic animal using an erythroid-specific promoter includes synthesizing an erythroid-specific globin gene promoter and globin gene locus control region and cloning the promoter and the globin gene locus control region and a gene encoding a non-erythroid protein into a vector to obtain a transgene; introducing the transgene in pronuclear embryos collected from a mammalian animal in vitro; transplanting the pronuclear embryos containing the transgene into oviduct of a female recipient of the mammalian animal to obtain a transgenic animal which then expresses the non-erythroid protein in progenitor cells of erythrocytes; and collecting blood from the transgenic animal and isolating the non-erythroid protein from the erythrocytes. Further disclosed are a transgenic animal expressing a non-erythroid protein in progenitor cells of erythrocytes and an isolated erythrocyte of a non-human transgenic animal containing a human non-erythroid protein encoded by a transgene.


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