The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 17, 2022
Filed:
May. 20, 2020
Applicant:
Twist Bioscience Corporation, South San Francisco, CA (US);
Inventors:
Rebecca Nugent, San Francisco, CA (US);
Siyuan Chen, San Mateo, CA (US);
Elian Lee, Union City, CA (US);
Nathan Raynard, San Mateo, CA (US);
Assignee:
Twist Bioscience Corporation, South San Francisco, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 9/00 (2006.01); C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6811 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1096 (2013.01); C12N 9/1252 (2013.01); C12N 9/93 (2013.01); C12N 15/1031 (2013.01); C12N 15/1093 (2013.01); C12P 19/34 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01); C12Q 2531/101 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/11002 (2013.01);
Abstract
Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.