The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 2022

Filed:

Feb. 16, 2017
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

George M. Church, Brookline, MA (US);

Seth Lawler Shipman, Boston, MA (US);

Jeffrey D. Macklis, Brookline, MA (US);

Jeffrey Matthew Nivala, Allston, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 9/22 (2006.01); G06N 3/12 (2006.01);
U.S. Cl.
CPC ...
C12N 15/113 (2013.01); C12N 9/22 (2013.01); G06N 3/123 (2013.01); C12N 2310/122 (2013.01); C12N 2310/20 (2017.05);
Abstract

This invention provides methods of altering a cell including providing the cell with a nucleic acid sequence encoding a Cas1 protein and/or a Cas2 protein of a CRISPR adaptation system, providing the cell with a CRISPR array nucleic acid sequence including a leader sequence and at least one repeat sequence, wherein the cell expresses the Cas1 protein and/or the Cas2 protein and wherein the CRISPR array nucleic acid sequence is within genomic DNA of the cell or on a plasmid. Also provided are methods and systems for nucleic acid storage and in vivo molecular recordings of events into a cell.


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