The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 26, 2022

Filed:

Apr. 29, 2020
Applicant:

Sangamo Therapeutics, Inc., Richmond, CA (US);

Inventors:

Michael C. Holmes, Richmond, CA (US);

Fyodor Urnov, Richmond, CA (US);

Assignee:

Sangamo Therapeutics, Inc., Brisbane, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/12 (2015.01); C12N 9/22 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C07K 14/435 (2006.01); C07K 14/47 (2006.01); C07K 14/715 (2006.01); C12N 9/14 (2006.01);
U.S. Cl.
CPC ...
A61K 35/12 (2013.01); C07K 14/43595 (2013.01); C07K 14/4717 (2013.01); C07K 14/715 (2013.01); C12N 9/14 (2013.01); C12N 9/22 (2013.01); C12N 15/62 (2013.01); C12N 15/85 (2013.01); C12Y 301/21004 (2013.01); A61K 2035/124 (2013.01); C07K 2319/00 (2013.01); C07K 2319/09 (2013.01); C07K 2319/81 (2013.01); C12N 2800/80 (2013.01);
Abstract

Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.


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