The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 2022
Filed:
Oct. 09, 2018
Applicant:
Affymetrix, Inc., Carlsbad, CA (US);
Inventor:
Michael H. Shapero, Sunnyvale, CA (US);
Assignee:
AFFYMETRIX, INC., Carlsbad, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6858 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6855 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6858 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C12Q 2525/179 (2013.01); C12Q 2535/125 (2013.01); C12Q 2565/501 (2013.01); C12Q 2565/514 (2013.01); C12Q 2565/537 (2013.01); C12Q 2600/156 (2013.01);
Abstract
The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general, the methods employ allele-specific extension of oligonucleotides that are complementary to one of the alleles at the 3' end of the oligonucleotide. The allele-specific oligonucleotides are resistant to proofreading activity from a polymerase and may be extended in an allele-specific manner by a DNA polymerase with a functional 3′ to 5′ exonuclease activity. The allele-specific oligonucleotides may be attached to a solid support such as a chip or a bead.