The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 12, 2022

Filed:

Aug. 17, 2018
Applicants:

Bontac Bio-engineering(shenzhen) Co., Ltd, Guangdong, CN;

Bontac Invitrolife Bio-technology(shenzhen) Co., Ltd, Guangdong, CN;

Jiangxi Bonzymes Biotechnology Co., Ltd., Jiangxi, CN;

Inventors:

Rongzhao Fu, Guangdong, CN;

Zhenwei Li, Guangdong, CN;

Min Wang, Guangdong, CN;

Xinglin Guo, Guangdong, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01); C12N 11/02 (2006.01); C12P 19/38 (2006.01);
U.S. Cl.
CPC ...
C12N 9/16 (2013.01); C12N 11/02 (2013.01); C12P 19/38 (2013.01); C12Y 301/03002 (2013.01);
Abstract

An acid phosphatase mutant, and method for preparing nicotinamide riboside by same. The mutant is a protein of the following (a), (b) or (c): (a) a protein, having an amino acid sequence shown in SEQ ID NO: 3; (b) a protein, derived from (a), having catalytic activity higher than an acid phosphatase parent having an amino acid sequence shown in SEQ ID NO: 2, obtained by substituting, deleting or adding several amino acids in the amino acid sequence shown in SEQ ID NO: 3, using nicotinamide mononucleotide as a substrate; (c) a protein, having catalytic activity higher than the acid phosphatase shown in SEQ ID NO: 2, having 90% or more homology with the amino acid sequence of the protein defined by (a) or (b), and using nicotinamide mononucleotide as a substrate. It is used to prepare nicotinamide riboside and the conversion rate can be 99%.


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