The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 05, 2022

Filed:

Dec. 23, 2020
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Long Liu, Wuxi, CN;

Jian Chen, Wuxi, CN;

Xueqin Lv, Wuxi, CN;

Guocheng Du, Wuxi, CN;

Jianghua Li, Wuxi, CN;

Jieying Deng, Wuxi, CN;

Ke Liu, Wuxi, CN;

Assignee:

JIANGNAN UNIVERSITY, Jiangsu, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 15/75 (2006.01); C12P 19/18 (2006.01);
U.S. Cl.
CPC ...
C12N 15/62 (2013.01); C12N 9/1051 (2013.01); C12N 9/1205 (2013.01); C12N 15/75 (2013.01); C12P 19/18 (2013.01);
Abstract

The present invention provides a recombinantwith improved 2'-fucosyllactose production, and a construction method thereof. In the present invention, a strain capable of efficiently synthesizing 2′-fucosyllactose is obtained by the fusion expression of the fucosyltransferase gene and the L-fucokinase/guanosine 5′-diphosphate-L-fucose pyrophosphorylase gene inBSGL-FF, the fermentation supernatant of which comprises a cumulative amount of 2′-fucosyllactose as high as 1.62 g/L, which is 55% higher than the amount achieved with the control strain. The construction method of the recombinantof the present invention is simple, and convenient to use, and thus has good application prospects.


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