The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2022
Filed:
Mar. 30, 2018
Applicant:
Global Life Sciences Solutions Operations Uk Ltd, Sheffield, GB;
Inventors:
Robert Scott Duthie, Schenectady, NY (US);
John Richard Nelson, Clifton Park, NY (US);
Anuradha Sekher, Hillsborough, NJ (US);
Assignee:
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD, Sheffield, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6848 (2013.01);
Abstract
Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.