The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 08, 2022

Filed:

Nov. 22, 2016
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Peter A. Carr, Medford, MA (US);

Brian Y. Chow, Cambridge, MA (US);

Joseph M. Jacobson, Newton, MA (US);

David W. Mosley, Cambridge, MA (US);

Christopher Emig, Napa, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6834 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6848 (2013.01);
Abstract

In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3' or 5′ protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3′ or 5′ associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.


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