The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2022
Filed:
Dec. 10, 2019
Dna Script Sas, Le Kremlin-Bicêtre, FR;
Xavier Godron, Paris, FR;
Adrian Horgan, Paris, FR;
Sylvain Gariel, Paris, FR;
Jeffrey Jeddeloh, Verona, WI (US);
Robert Nicol, Cambridge, MA (US);
Thomas Ybert, Paris, FR;
DNA Script SAS, Le Kremlin-Bicêtre, FR;
Abstract
The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3'-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.