The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 08, 2022

Filed:

May. 13, 2016
Applicant:

Zumutor Biologies, Inc., Woburn, MA (US);

Inventors:

Sohang Chatterjee, Bangalore, IN;

Kavitha Iyer Rodrigues, Bangalore, IN;

Maloy Ghosh, Bangalore, IN;

Sunit Maity, Bangalore, IN;

Divya Unnikrishnan, Bangalore, IN;

Jahnabi Hazarika, Bangalore, IN;

Yogendra Manjunath Bangalore Muniraju, Bangalore, IN;

Sathyabalan Murugesan, Bangalore, IN;

Pavithra Mukunda, Kundapur, IN;

Bhargav Prasad, Chennai, IN;

Veeresha Kamanagowda, Bangalore, IN;

Sanghamitra Bhattacharjee, Bangalore, IN;

Pravin Kumar Dakshinamurthy, Chennai, IN;

Vivek Halan, Aravenu, IN;

Sankaranarayanan Srinivasan, Bangalore, IN;

Anuradha Hora, Sitapur, IN;

Bairavabalakumar Natarajan, Chennai, IN;

Karthika Nair, Bangalore, IN;

Aswini Thanigaivel, Chennai, IN;

Amol Maliwalave, Bangalore, IN;

Bharath Ravindra Shenoy, Bangalore, IN;

Sahana Bhima Rao, Bangalore, IN;

Subhra Prakash Chakrabarty, Bangalore, IN;

Ashvini Kumar Dubey, Bangalore, IN;

Amir Khan, Aligarh, IN;

Ankurina Sharma, Bangalore, IN;

Assignee:

Zumutor Biologics Inc., San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C07K 16/32 (2006.01); C12N 15/113 (2010.01); C07K 16/06 (2006.01); C12N 9/22 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C07K 16/32 (2013.01); C07K 16/065 (2013.01); C12N 9/22 (2013.01); C12N 15/1137 (2013.01); C12N 15/907 (2013.01); C07K 2317/14 (2013.01); C07K 2317/41 (2013.01); C07K 2317/73 (2013.01); C07K 2317/732 (2013.01); C07K 2317/76 (2013.01); C12N 2310/10 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C12Y 204/01068 (2013.01);
Abstract

The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.


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