The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2022
Filed:
Dec. 14, 2018
Jiangnan University, Wuxi, CN;
Jiangnan University, Wuxi, CN;
Abstract
The present invention discloses a method for promotingto synthesize surfactin based on multi-gene synergy, and belongs to the field of genetic engineering. Firstly,is enabled to obtain the ability to synthesize surfactin by integrant expression of sfp protein derived from a high-yield strain, on this basis, by knocking out genes associated with a competitive pathway, overexpressing genes associated with the surfactin tolerance of, strengthening genes associated with a branched chain fatty acid synthesis pathway or improving the intracellular srfA gene transcription level, the synergy among genes is realized, and systemic metabolic engineering transformation is performed on the, thereby greatly improving the ability ofgenetically engineered bacteria to synthesize surfactin. Compared with a starting strain168, the amount of extracellular accumulation of surfactin of thegenetically engineered bacteria increases from 0 g/L to 12.8 g/L. The present invention obtains the recombinantwith significantly improved ability to synthesize surfactin by multi-gene cooperative transformation of, and has a good application prospect.