The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 18, 2022
Filed:
Oct. 17, 2017
New England Biolabs, Inc., Ipswich, MA (US);
Bo Yan, Ipswich, MA (US);
Laurence Ettwiller, Ipswich, MA (US);
Ira Schildkraut, Ipswich, MA (US);
George Tzertzinis, Ipswich, MA (US);
Ivan R. Correa, Jr., Ipswich, MA (US);
Nan Dai, Ipswich, MA (US);
Madalee G. Wulf, Ipswich, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Provided herein is a method for making an cDNA library, comprising adding an affinity tag-labeled GMP to the 5' end of targeted RNA species in a sample by optionally decapping followed by incubating the sample with an affinity tag-labeled GTP and a capping enzyme, enriching for RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag, reverse transcribing the enriched RNA to produce a population of cDNAs, and adding a tail to the 3′ end of the population of cDNAs using a terminal transferase, to produce an cDNA library.