The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 28, 2021
Filed:
Feb. 02, 2017
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
George M. Church, Brookline, MA (US);
Luhan Yang, Somerville, MA (US);
Margo R. Monroe, Boston, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01); C12N 7/00 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/113 (2013.01); C12N 7/00 (2013.01); C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 15/63 (2013.01); A61K 48/00 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01); C12N 2800/80 (2013.01);
Abstract
Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.