The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 21, 2021
Filed:
Jun. 08, 2020
Jiangnan University, Wuxi, CN;
Zhiming Rao, Wuxi, CN;
Peifeng Duan, Wuxi, CN;
Jianing Zhang, Wuxi, CN;
Xian Zhang, Wuxi, CN;
Taowei Yang, Wuxi, CN;
Meijuan Xu, Wuxi, CN;
Minglong Shao, Wuxi, CN;
Yu Liu, Wuxi, CN;
Ziwei Wang, Wuxi, CN;
Yan Chen, Wuxi, CN;
Qi Chen, Wuxi, CN;
Jiangnan University, Wuxi, CN;
Abstract
The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from, and subjected to site-directed mutagenesis to improve the enzyme activity of sucrose phosphorylase. The mutant is expressed inand used as a whole cell catalyst to produce 2-O-α-D-glycerol glucoside. At a 5 L fermentation tank level, a large quantity of 2-O-α-D-glycerol glucoside can be produced efficiently in a short time, which is conducive to expanding the prospect of industrial application of sucrose phosphorylase for the production of 2-O-α-D-glycerol glucoside and realizing its large-scale industrial application.