Method for labeling specifically living bacteria comprising the use of modified non endogenous monosaccharide compounds

Abstract

The invention relates to a method for labeling specifically living bacteria, comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into the envelope of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the envelope of the bacteria with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group with said second reactive group, characterized in that said modified monosaccharide compound has the following formula (I), or a salt thereof: Wherein —A, B and C can be independently H, OH, NH, OH and NHbeing substituted or not by protecting groups thereof and —D is an alkyl chain in Cto C, each carbon being substituted or not by OH or NH, OH and NHbeing substituted or not by protecting groups thereof and —at least one of A, B, C or D groups is substituted by a said first reactive group.