The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 07, 2021

Filed:

Nov. 15, 2017
Applicants:

Tokyo University of Science, Tokyo, JP;

National University Corporation Chiba University, Chiba, JP;

Jeol Ltd., Tokyo, JP;

Inventors:

Tatsuya Higashi, Kashiwa, JP;

Shoujiro Ogawa, Kashiwa, JP;

Fumio Nomura, Chiba, JP;

Mamoru Satoh, Chiba, JP;

Masaki Takiwaki, Chiba, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/82 (2006.01); G01N 30/72 (2006.01);
U.S. Cl.
CPC ...
G01N 33/82 (2013.01); G01N 30/72 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01);
Abstract

Provided is a method for quantifying vitamin D, with the vitamin D contained in a biological sample being derivatized with a derivatization reagent and being measured with a mass spectrometer, the method including, a derivatization step of derivatizing n number of samples by using n types of 4-(4'-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) isotopologues respectively as derivatization reagents, a mixing step of mixing the n types of derivatization samples obtained in the derivatization step, and a quantitative analysis step of subjecting each of the n types of vitamin D derivatives contained in the mixed sample obtained in the mixing step to quantitative analysis using a mass spectrometer.


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