The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 07, 2021

Filed:

Aug. 10, 2017
Applicant:

University of Maryland, College Park, College Park, MD (US);

Inventors:

Lai-Xi Wang, Ellicott City, MD (US);

Chao Li, College Park, MD (US);

Shilei Zhu, Halethorpe, MD (US);

Assignee:

UNIVERSITY OF MARYLAND, COLLEGE PARK, College Park, MD (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/24 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01127 (2013.01); C07K 2319/21 (2013.01); C07K 2319/50 (2013.01);
Abstract

The present invention provides for novel fucosidase mutants that server as fuco-ligases for core fucosylation of a range of biological glycopeptides and glycoproteins including intact therapeutic antibodies. Several mutants with mutation at the general acid/base residue E274 of theα1,6-fucosidase, including E274A, E274S, and E274G, were able to efficiently fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins. The site specific mutants enable the transfer of fucose to a core GlcNAc-Asn residue and useful for drug delivery and vaccine development.


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