The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 09, 2021

Filed:

Mar. 13, 2017
Applicant:

Fujirebio Inc., Tokyo, JP;

Inventors:

Noriko Katagiri, Tokyo, JP;

Shintaro Yagi, Tokyo, JP;

Assignee:

FUJIREBIO INC., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/88 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/88 (2013.01); G01N 33/54393 (2013.01);
Abstract

Disclosed is a method of measuring the prostaglandin E main urinary metabolite (PGE-MUM), in which a mixture solution of a urine sample treated with alkali can directly be subjected to an antigen-antibody reaction system in an immunoassay of PGE-MUM, without neutralization and dilution followed by dispensation. The method of measuring PGE-MUM includes the steps of: a) mixing a urine sample with an alkaline aqueous solution, and b) subjecting the mixture solution resulting from a) to an immunoassay using a bicyclo PGE-MUM-immobilized or anti-bicyclo PGE-MUM antibody-immobilized solid phase to measure PGE-MUM in the urine sample, wherein the immunoassay is performed in a weakly-acidic basal buffer solution in the presence of a second pH buffering agent which exerts a buffering effect in the basic range and is different from the pH buffering agent contained in the basal buffer solution, and in the presence of a cationic surfactant.


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