The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 09, 2021
Filed:
Apr. 17, 2020
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Assignee:
HOFFMANN-LA ROCHE INC., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/20 (2006.01); C07K 1/36 (2006.01); C07K 14/54 (2006.01);
U.S. Cl.
CPC ...
C07K 1/165 (2013.01); C07K 1/18 (2013.01); C07K 1/20 (2013.01); C07K 1/36 (2013.01); C07K 14/5443 (2013.01); C07K 2319/30 (2013.01);
Abstract
Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide in monomeric form and in aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide in monomeric form.