The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 02, 2021

Filed:

Jun. 11, 2019
Applicants:

Yi Xiao, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Juan Canoura, Hialeah Gardens, FL (US);

Yingzhu Liu, Miami, FL (US);

Inventors:

Yi Xiao, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Juan Canoura, Hialeah Gardens, FL (US);

Yingzhu Liu, Miami, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/94 (2006.01); C12N 15/115 (2010.01); G01N 33/58 (2006.01); C12Q 1/6811 (2018.01);
U.S. Cl.
CPC ...
G01N 33/946 (2013.01); C12N 15/115 (2013.01); C12Q 1/6811 (2013.01); G01N 33/582 (2013.01); C12Y 301/15 (2013.01); C12Y 301/16 (2013.01);
Abstract

The subject invention provides methods, assays, and products for detecting small-molecule targets in a complex sample in both clinical and field settings. The subject invention provides aptamer-based sensors and methods of use thereof. The subject invention provides exonuclease-based methods for generating structure-switching aptamers from fully folded or pre-folded aptamers and developing aptamer-based sensors for small-molecule detection. The method for detecting one or more small-molecule targets in a sample comprises contacting the sample with one or more aptamer-based sensor selective for each of the small-molecule targets, and detecting the small-molecule target in the sample.


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