The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Dec. 28, 2020
Applicants:

Nutrition & Health Research Institute, Cofco Corporation, Beijing, CN;

Cofco (Jilin) Bio-chemical Technology Co., Ltd, Changchun, CN;

Cofco Biotechnology Co., Ltd., Bengbu, CN;

Tsinghua University, Beijing, CN;

Inventors:

Yi Tong, Beijing, CN;

Yi Li, Beijing, CN;

Fang Tian, Beijing, CN;

Weiqiang Zhou, Beijing, CN;

Yuanheng Guo, Beijing, CN;

Guoqiang Chen, Beijing, CN;

Dayong Li, Changchun, CN;

Haijun Liu, Changchun, CN;

Kejia Xu, Beijing, CN;

Anni Liu, Beijing, CN;

Bo Chen, Beijing, CN;

Tai An, Beijing, CN;

Xiaoyan Wang, Beijing, CN;

Chao Peng, Beijing, CN;

Xuemei Shen, Beijing, CN;

Jin Tao, Changchun, CN;

Lida Wu, Changchun, CN;

Kai Yang, Beijing, CN;

Qiong Wu, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C02F 3/12 (2006.01); C02F 3/34 (2006.01); C12M 1/00 (2006.01); C12N 1/02 (2006.01); C12N 1/06 (2006.01); C12P 7/62 (2006.01); C12M 1/26 (2006.01); C08G 63/90 (2006.01); B01D 37/02 (2006.01); B01D 39/08 (2006.01); B01D 61/00 (2006.01); C02F 1/28 (2006.01); C02F 1/72 (2006.01);
U.S. Cl.
CPC ...
C02F 3/1221 (2013.01); B01D 37/02 (2013.01); B01D 39/08 (2013.01); B01D 61/00 (2013.01); C02F 1/28 (2013.01); C02F 1/72 (2013.01); C02F 3/1263 (2013.01); C02F 3/34 (2013.01); C08G 63/90 (2013.01); C12M 29/00 (2013.01); C12M 33/14 (2013.01); C12N 1/02 (2013.01); C12N 1/06 (2013.01); C12N 1/066 (2013.01); C12P 7/62 (2013.01); B01D 2239/0471 (2013.01); C02F 2209/001 (2013.01); C02F 2209/105 (2013.01); C12N 2500/05 (2013.01); C12N 2500/12 (2013.01); C12N 2500/16 (2013.01); C12N 2500/34 (2013.01);
Abstract

A method for efficiently producing PHA comprising: inoculating PHA fermentation strains into a fermentation medium for fermentation under the condition of being capable of producing PHA through fermentation; subjecting the fermentation broth to a solid-liquid separation to obtain fermentation supernatant and thallus precipitate; breaking the cell walls of the thallus precipitate, and subjecting the wall-broken products to a plate and frame filtration to prepare PHA; pre-coating a filter cloth for the plate and frame filtration with a PHA layer; at least part of the water of the fermentation medium is PHA process wastewater. The method utilizes the PHA process wastewater as at least part of the water of the fermentation medium, and filters and separates the broken thallus with the plate and frame filtration equipment pre-coated with PHA layer to prepare PHA, thereby recycling the high-salt wastewater, reducing costs, and potentially separating PHA on a large scale for industrial production.


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