The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 2021
Filed:
Sep. 28, 2018
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Timothy Craig, Campbell, CA (US);
Corissa Harris, Santa Clara, CA (US);
Matt Dipetro, Gilroy, CA (US);
Mark Ambroso, San Diego, CA (US);
Yaozhong Zou, Sunnyvale, CA (US);
Marshall Porter, Petaluma, CA (US);
Issa Delkaninia, San Jose, CA (US);
Alexxa Noble, San Jose, CA (US);
James Fairman, Sunnyvale, CA (US);
Wiseley Wu, Newark, CA (US);
Kapil Bajaj, Newark, CA (US);
Giovanni Bellesia, San Jose, CA (US);
Seong-Ho Shin, Oakland, CA (US);
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Abstract
Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.